Ijeli ye-hyaluronate ye-sodium ene-Cross-Linked yokuhlinzwa


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Okusunguliwe kwamanje kuhlobene nejeli ye-sodium hyaluronate exhunywe ngokuphambana yokugcwalisa izicubu zokuhlinzwa kwepulasitiki kanye nendlela yokuyilungiselela.Isixazululo se-alkaline se-sodium hyaluronate sisabela ne-alkane yeketango elide eliqukethe i-epoxy group kanye ne-agent exhumanisayo equkethe iqembu le-epoxy amahora angu-2˜5 ku-35° C.˜50° C. ukuze kukhiqizwe i-hyaluronate ye-sodium exhunywe ngokuphambanayo, bese iyawashwa, gelled futhi inzalo, ukulungiselela ijeli.Phakathi kwazo, isilinganiso se-molar ye-sodium hyaluronate:i-ejenti exhumanisayo equkethe iqembu le-epoxy:uchungechunge olude lwe-alkane oluqukethe iqembu le-epoxy ngu-10:4˜1:1˜4;inani lama-athomu ekhabhoni yaleli ketango elide le-alkane eliqukethe iqembu le-epoxy liyi-6 kuye ku-18. Ijeli elungiselelwe ekusungulweni kwamanje, ngakolunye uhlangothi, ingathuthukisa ngempumelelo ukumelana ne-enzymolysis ukuze izinze, futhi ngakolunye uhlangothi, igcine i-biocompatibility enhle kakhulu ye-sodium hyaluronate ngaphandle kokuphazamisa ukujova kwayo.

Ijeli ye-Crosslinked sodium hyaluronate yokuhlinzwa kwepulasitiki yokuhlinzwa kanye nendlela yokulungiselela:

Ukusungulwa kuhlobene nendlela yokulungiselela ijeli ye-sodium hyaluronate exhunywe ngokuphambana yokuhlinzwa kwepulasitiki yokuhlinzwa, ebonakala ngokuhlanganisa lezi zinyathelo ezilandelayo:
(1) I-sodium hyaluronate powder eyomile yahlakazwa ku-10?wt%~20?Isixazululo esixubile esakhiwe yi-wt% ye-sodium hydroxide aqueous solution kanye ne-acetone yasetshenziselwa ukuthola ukumiswa okuyisisekelo kwe-sodium hyaluronate, bese kuba yi-agent e-crosslinking 1,4?I-Butanediol diglycidyl ether BDDE ixutshwe ngokulinganayo ukuze kutholwe izinto zokusabela, ukuze kuqalwe ukusabela ukukhiqiza i-hyaluronate ye-sodium exhumene nesiphambano;Ngaphansi kwesimo esinyakazayo, ukusabela kuqedwa ngemva kokuba impahla yokusabela igcinwe ku-35 ℃ ~ 50 ℃ amahora angu-5 ~ 8, kanye nenani le-pH lezinto ezixutshwe noketshezi oluqinile ngemva kokusabela kulungiswa ku-7 nge-hydrochloric acid egxilile;Lapho ukuhlushwa kwe-sodium hyaluronate ku-reaction material kungu-2?I-Wt% ~ 5wt%, isilinganiso sobuningi be-agent e-crosslinking ku-sodium hyaluronate ngu (1: 1.3) ~ (1: 1.8);
(2) Hlunga ingxube eqinile newuketshezi ye-pH=7 ngemva kokusabela ukuze ususe uketshezi.Izinto ezisele zigezwa nge-acetone ku-GC?MS ukuthola okuqukethwe kwe-BDDE ngaphansi kuka-2ppm.Izinto ezisele zifaka impushana emhlophe nejeli esobala, bese izinto ezomisiwe zomisiwe ukuze kutholwe impushana emhlophe eyomile engancibiliki emanzini, okungukuthi, impushana exhumene ne-sodium hyaluronate.
(3) Ukusefa nokuhlukanisa impushana ye-sodium hyaluronate ephambanisiwe etholwe ngokumisa nge-vacuum esinyathelweni ② nokuqoqa impushana ehlungiwe;
(4) Ukwengeza amanzi angcolile ku-powder ehlungiwe eqoqwe esinyathelweni sesi-3, ukuze i-sodium hyaluronate powder exhunywe ngokuphambana ivuvuke ngokugcwele futhi ihlanzwe amahora angu-6 ~ 10 ekamelweni lokushisa kusuka ku-15 kuya ku-35 degrees, futhi izinhlayiya zejeli ziqoqwe ukuze thola ijeli ye-sodium hyaluronate ehlanganisiwe.
(5) Isigcinalwazi se-isotonic se-PBS sengezwe kujeli eqoqwe esinyathelweni sesi-4 futhi sahlanzwa amahora angu-6~10 ekamelweni lokushisa ukusuka ku-15 kuya ku-35 degrees.Ngemva kokuhlunga, i-PBS yakhishwa futhi ijeli yaqoqwa.Ijeli yahlolwa ngokulandelana kusetshenziswa ukucaciswa kokuqala kanye nokucaciswa kwesibili kwesikrini.Kutholwe osayizi abahlukene aba-3 bamajeli, kwathi amajeli ama-3 afakwa inzalo futhi agcwaliswa ngesipetu esilahlwa inzalo ngaphambili.


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